nec8 cells Search Results


nec8 cells  (JCRB Cell Bank)
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JCRB Cell Bank nec8 cells
Human cell lines <t>NEC8</t> and HuTu80 but not HCT-8 supported the replication of HuSaV GI.1 and GII.3 in the presence of bile acids in the cell culture medium. HuSaV GI.1 (AK20) or GII.3 (AK11) positive stool suspension was inoculated onto the cell monolayers in the presence of CA, GlyCA, DCA, GCDCA, or Bile, or in the absence of bile acids (Mock). After removing the inoculum and washing the cell monolayers at 1 dpi, we collected the culture supernatants at 7 dpi. The optimal concentration of bile acid for each cell line, as indicated in Materials and Methods, was present throughout the culture period. The HuSaV RNA was measured by RT-PCR followed by agarose gel electrophoresis. The GI.1 inoculum and water was included as positive and negative controls, respectively, for HCT-8 cells. The results were confirmed in an independent experiment, and pictures of a representative experiment are shown.
Nec8 Cells, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nec8 cells  (BioResource International Inc)
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BioResource International Inc nec8 cells
Human cell lines <t>NEC8</t> and HuTu80 but not HCT-8 supported the replication of HuSaV GI.1 and GII.3 in the presence of bile acids in the cell culture medium. HuSaV GI.1 (AK20) or GII.3 (AK11) positive stool suspension was inoculated onto the cell monolayers in the presence of CA, GlyCA, DCA, GCDCA, or Bile, or in the absence of bile acids (Mock). After removing the inoculum and washing the cell monolayers at 1 dpi, we collected the culture supernatants at 7 dpi. The optimal concentration of bile acid for each cell line, as indicated in Materials and Methods, was present throughout the culture period. The HuSaV RNA was measured by RT-PCR followed by agarose gel electrophoresis. The GI.1 inoculum and water was included as positive and negative controls, respectively, for HCT-8 cells. The results were confirmed in an independent experiment, and pictures of a representative experiment are shown.
Nec8 Cells, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nec8+cells/human+ec+cell+line+nec8/pm39660536-132-23-29
Average 90 stars, based on 1 article reviews
nec8 cells - by Bioz Stars, 2026-10
90/100 stars
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Human cell lines NEC8 and HuTu80 but not HCT-8 supported the replication of HuSaV GI.1 and GII.3 in the presence of bile acids in the cell culture medium. HuSaV GI.1 (AK20) or GII.3 (AK11) positive stool suspension was inoculated onto the cell monolayers in the presence of CA, GlyCA, DCA, GCDCA, or Bile, or in the absence of bile acids (Mock). After removing the inoculum and washing the cell monolayers at 1 dpi, we collected the culture supernatants at 7 dpi. The optimal concentration of bile acid for each cell line, as indicated in Materials and Methods, was present throughout the culture period. The HuSaV RNA was measured by RT-PCR followed by agarose gel electrophoresis. The GI.1 inoculum and water was included as positive and negative controls, respectively, for HCT-8 cells. The results were confirmed in an independent experiment, and pictures of a representative experiment are shown.

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Human sapovirus propagation in human cell lines supplemented with bile acids

doi: 10.1073/pnas.2007310117

Figure Lengend Snippet: Human cell lines NEC8 and HuTu80 but not HCT-8 supported the replication of HuSaV GI.1 and GII.3 in the presence of bile acids in the cell culture medium. HuSaV GI.1 (AK20) or GII.3 (AK11) positive stool suspension was inoculated onto the cell monolayers in the presence of CA, GlyCA, DCA, GCDCA, or Bile, or in the absence of bile acids (Mock). After removing the inoculum and washing the cell monolayers at 1 dpi, we collected the culture supernatants at 7 dpi. The optimal concentration of bile acid for each cell line, as indicated in Materials and Methods, was present throughout the culture period. The HuSaV RNA was measured by RT-PCR followed by agarose gel electrophoresis. The GI.1 inoculum and water was included as positive and negative controls, respectively, for HCT-8 cells. The results were confirmed in an independent experiment, and pictures of a representative experiment are shown.

Article Snippet: For HuSaV culture trials, we used three human cell lines: 1) human testicular embryonal carcinoma-derived cell line, NEC8 cells (Japanese Collection of Research Bioresources [JCRB] 0250); 2) human duodenum carcinoma derived cell line, HuTu80 cells (American Tissue Culture Collection [ATCC] #HTB-40); and 3) human ileocecal adenocarcinoma derived cell line, HCT-8 (HRT-18) cells (ATCC #CCL-244).

Techniques: Cell Culture, Concentration Assay, Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis

Growth kinetics of HuSaV GI.1 and GII.3 in NEC8 and/or HuTu80 cells. HuSaV GI.1 (AK20) or GII.3 (AK11) positive stool suspension was inoculated onto NEC8 or HuTu80 cell monolayers. After removing the inoculum and washing the cell monolayers at 1 dpi, we collected supernatants at 1, 3, 5, and 7 dpi or 1, 3, 5, 7, 10, and 14 dpi. GlyCA (500 µM for NEC8 and 1,000 µM for HuTu80) was present throughout the culture period. The SaV RNA was measured by RT-qPCR. Dotted line indicates the lowest quantification limit. Data represent the values of two independent experiments using cells at different passage numbers for each time point. Error bars indicate SDs.

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Human sapovirus propagation in human cell lines supplemented with bile acids

doi: 10.1073/pnas.2007310117

Figure Lengend Snippet: Growth kinetics of HuSaV GI.1 and GII.3 in NEC8 and/or HuTu80 cells. HuSaV GI.1 (AK20) or GII.3 (AK11) positive stool suspension was inoculated onto NEC8 or HuTu80 cell monolayers. After removing the inoculum and washing the cell monolayers at 1 dpi, we collected supernatants at 1, 3, 5, and 7 dpi or 1, 3, 5, 7, 10, and 14 dpi. GlyCA (500 µM for NEC8 and 1,000 µM for HuTu80) was present throughout the culture period. The SaV RNA was measured by RT-qPCR. Dotted line indicates the lowest quantification limit. Data represent the values of two independent experiments using cells at different passage numbers for each time point. Error bars indicate SDs.

Article Snippet: For HuSaV culture trials, we used three human cell lines: 1) human testicular embryonal carcinoma-derived cell line, NEC8 cells (Japanese Collection of Research Bioresources [JCRB] 0250); 2) human duodenum carcinoma derived cell line, HuTu80 cells (American Tissue Culture Collection [ATCC] #HTB-40); and 3) human ileocecal adenocarcinoma derived cell line, HCT-8 (HRT-18) cells (ATCC #CCL-244).

Techniques: Quantitative RT-PCR